FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53996096
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC314550.019.3502719
GATCGGA347750.018.1992511
AGAGCGT346950.017.835638
ATCGGAA374200.016.9267792
TCGGAAG396950.015.9544553
CGGAAGA420400.015.1162484
AAGAGCG427000.014.8484427
CGCCGTA117500.013.29607955-59
TCGCCGT132900.011.94075555-59
GCCGTAT143400.011.0462255-59
GTCGCCG156650.010.01012655-59
CGTATCA160750.09.80961360-64
TGGTCGC169900.09.46793650-54
GTGGTCG181250.09.01896750-54
GAAGAGC778000.08.8015546
AGGTCGT180550.08.64488235-39
CCGTATC159350.07.712901660-64
GGTCGTG209800.07.688304435-39
TCTCGGT226350.07.33081645-49
GGTCGCC182000.06.856057655-59
Produced by FastQC (version 0.11.5)