FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73040353
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC26800.021.638146
CGACGCT29600.019.836187
CACGACG32150.017.8119375
TATGCCG113000.011.22773745-49
GATCGGA329800.011.1826711
TCGGAAG375250.010.025633
ATCGGAA369250.010.0237642
CTCGTAT132900.09.61137840-44
CGGAAGA405550.09.4553214
TCTCGTA130400.09.06180540-44
CGTATGC137250.08.78969145-49
ACACGAC74150.08.309464
ATGCCGT162450.07.818925445-49
GACGCTC87150.07.73535548
TACACGA84050.07.41695743
GCCGTCT169100.07.16858350-54
CGTGTAG180000.07.02499730-34
CCGTGTA185150.06.954908430-34
ATCTCGT173200.06.872740340-44
GTCACCG193950.06.56416625-29
Produced by FastQC (version 0.11.5)