FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71674195
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143800.011.92306845-49
GATCGGA503800.011.3002971
TCGGAAG537400.010.7813453
ATCGGAA535200.010.7308212
CGGAAGA554600.010.4413974
CGTATGC164950.010.21855745-49
CTCGTAT169050.010.0909240-44
TCTCGTA165350.09.85216340-44
ATGCCGT191600.09.06955245-49
GCCGTCT192700.08.77739450-54
ATCTCGT199150.08.29648340-44
CGTCTTC245500.07.26746650-54
CCGTCTT248900.07.127430450-54
AGAGCAC926500.06.6245038
TCGTATG191000.06.389117245-49
GAGCACA983750.06.3421099
TGCCGTC193700.06.217959450-54
AGATCGG502550.06.1155844145
GAAGAGC1047600.05.9555656
GTATGCC346750.05.43779745-49
Produced by FastQC (version 0.11.5)