FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17308270
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACCACCGGTGTAGATCT556390.32145904818910265Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC189500.048.316279
AGAGCGT203200.045.3800778
GATCGGA230050.041.0870321
AAGAGCG230100.040.325187
ATCGGAA236400.040.076152
TCGGAAG236950.039.8910063
CGGAAGA239100.039.3807224
GAAGAGC348400.027.1112336
CGCCGTA84150.023.96455855-59
TCGCCGT89050.022.48309955-59
GCCGTAT93200.021.43530755-59
GTCGCCG95050.020.89608255-59
TGGTCGC99600.020.18916150-54
CGTATCA100250.019.90097660-64
GGAAGAG482300.019.8984765
GTGGTCG103500.019.68054650-54
CGGTGTA104200.019.24537735-39
ACCGGTG108450.018.45106735-39
CACCGGT108500.018.1351935-39
CCGTATC98650.017.9309460-64
Produced by FastQC (version 0.11.5)