FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28952701
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACCACCGGTGTAGATCT868730.30005145288517293Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC303950.046.2965479
AGAGCGT328500.042.9028438
GATCGGA365400.039.478051
ATCGGAA376550.038.2949752
TCGGAAG379250.038.058543
AAGAGCG377450.037.895697
CGGAAGA392750.036.7503554
GAAGAGC576950.025.1438126
CGCCGTA132550.023.17869855-59
TCGCCGT142750.021.61390955-59
GCCGTAT150150.020.45212455-59
GTCGCCG148750.020.4204355-59
TGGTCGC158950.019.41128550-54
CGTATCA160000.019.1385160-64
GTGGTCG167450.018.55582250-54
GGAAGAG809400.018.3168835
CGGTGTA168050.018.23906735-39
ACCGGTG176150.017.3345235-39
CACCGGT177650.016.95147335-39
CCGTATC161000.016.74124360-64
Produced by FastQC (version 0.11.5)