FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72240449
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT853700.1181747915215754TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA659350.023.1589581
ATCGGAA676350.022.5984952
TCGGAAG686750.022.2140123
CGGAAGA706750.021.616154
CGGCGAC55000.016.1138531
TATGCCG223950.015.03364945-49
AGAGCAC1063200.014.4496758
GAGCACA1132500.013.5782679
CGTATGC252500.013.26492745-49
GAAGAGC1197300.013.1278546
CTCGTAT250250.013.12392140-44
TCTCGTA246250.013.10167340-44
ATGCCGT264400.012.41575145-49
GCGACCA75650.012.1665753
GCCGTCT281900.011.98434150-54
TCGTATG265200.011.5530845-49
GGCGACC82550.011.4130232
CTTACGG303050.011.17759430-34
TTACGGT300300.011.10956230-34
ATCTCGT291250.011.05249840-44
Produced by FastQC (version 0.11.5)