FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72235102
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT828880.11474753645395282TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA628050.022.7086831
ATCGGAA647450.022.0371322
TCGGAAG667400.021.389263
CGGAAGA682600.020.954874
CGGCGAC56050.017.7586841
TATGCCG218450.014.61637145-49
AGAGCAC1042600.013.8997788
GCGACCA76750.013.60255053
CGTATGC237050.013.29823245-49
GAGCACA1113300.013.0495069
TCTCGTA232800.013.03590840-44
CTCGTAT236550.013.031527540-44
GAAGAGC1168400.012.5585346
ATGCCGT260200.011.90889745-49
CGACCAC88550.011.8714464
GGCGACC89350.011.7654812
GCCGTCT276450.011.34459750-54
TCGTATG258550.011.25021345-49
CTTACGG289600.010.98545630-34
TTACGGT283850.010.90148330-34
Produced by FastQC (version 0.11.5)