FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14010670
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65750.018.3080711
ATCGGAA70200.017.2471522
TCGGAAG70800.016.9985873
CGGAAGA77550.015.6118954
TATGCCG24450.012.21606245-49
CTCGTAT27400.010.9539840-44
TCTCGTA28300.010.400681540-44
CGTATGC27950.010.01194445-49
ATGCCGT32200.09.23083245-49
GCCGTCT34500.08.53174450-54
TCGTATG31200.08.22548645-49
ATCTCGT36050.08.20497140-44
AGAGCAC158550.07.955778
TGCCGTC34750.07.510668350-54
GAGCACA167500.07.44411239
GAAGAGC179000.07.1684576
CGTCTTC43150.06.922250750-54
CCGTCTT44300.06.644360550-54
AGATCGG64100.05.7678986145
CGTCTGA54250.05.639148715-19
Produced by FastQC (version 0.11.5)