FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14010670
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC65500.018.150329
GATCGGA75600.016.1870171
AGAGCGT73850.015.8036418
ATCGGAA80200.015.1869082
TCGGAAG82650.014.4719663
CGGAAGA88350.013.7844414
AAGAGCG89100.013.3429717
CGCCGTA21300.013.13724655-59
TCGCCGT24950.011.27347755-59
GCCGTAT28750.09.93470655-59
GTCGCCG29000.09.74907155-59
TGGTCGC32100.08.94346950-54
CGTATCA34350.08.48427160-64
GTGGTCG35800.08.10014650-54
CCGTATC32550.07.52802360-64
GGTCGCC35200.07.002174455-59
GAAGAGC184450.06.9956956
TCTCGGT48800.06.35802445-49
CGGTGGT51950.06.02856750-54
CTCGGTG52850.05.788494645-49
Produced by FastQC (version 0.11.5)