FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63533917
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA321800.024.3584481
ATCGGAA340450.023.1907962
TCGGAAG351500.022.4611193
CGGAAGA378850.020.6481724
TATGCCG125950.013.2272445-49
CTCGTAT141500.011.43572140-44
TCTCGTA140250.011.38256940-44
CGTATGC146000.011.31144845-49
AGAGCAC717100.011.2218788
GAGCACA789500.010.4407249
ATGCCGT160400.09.98861645-49
GAAGAGC822550.09.9505826
TGCCGTC161050.09.91233650-54
TCGTATG165600.09.75376445-49
GCCGTCT172350.09.61577650-54
ATCTCGT174250.09.15325840-44
CGTCTTC206600.08.25327850-54
CCGTCTT212350.08.0161450-54
AAGAGCA1149150.07.31184537
AATCTCG266950.06.6482540-44
Produced by FastQC (version 0.11.5)