FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63533917
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC330950.023.4147059
GATCGGA370300.021.155611
AGAGCGT369250.021.1235968
ATCGGAA388100.020.2094862
TCGGAAG401800.019.5385133
CGGAAGA428350.018.37794
AAGAGCG433450.018.3121747
CGCCGTA116000.013.88585755-59
TCGCCGT132550.012.31615855-59
GCCGTAT148900.011.10009755-59
GTCGCCG148800.010.99063655-59
TGGTCGC169100.09.7652250-54
GAAGAGC864850.09.6806826
CCGTATC166850.09.3756260-64
CGTATCA179250.09.26894460-64
GTGGTCG183200.09.13234450-54
GGTCGCC177950.08.75029955-59
TCTCGGT235700.07.24583745-49
TCGGTGG235700.06.784550-54
CTCGGTG260050.06.57851745-49
Produced by FastQC (version 0.11.5)