FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69579291
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC361600.018.9999359
AGAGCGT417350.016.6702488
CGCCGTA103050.016.19261755-59
GATCGGA446350.015.8391571
ATCGGAA469900.015.2389212
TCGGAAG481000.014.7358643
TCGCCGT117350.014.31825555-59
CGGAAGA505050.014.1920064
AAGAGCG496650.014.0967137
GTCGCCG125800.013.26430355-59
GCCGTAT142300.011.94022555-59
TGGTCGC162050.010.5747250-54
GTGGTCG172050.010.06120950-54
CGTATCA171900.09.95201760-64
GGTCGCC160100.08.84693855-59
CCGTATC165250.08.75576460-64
TCTCGGT225450.07.92155745-49
GAAGAGC1006350.07.41787776
CGGTGGT256200.07.028181650-54
CTCGGTG255300.06.932900445-49
Produced by FastQC (version 0.11.5)