FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68486029
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA402850.015.1980811
TCGGAAG430050.014.4973283
ATCGGAA425450.014.4677372
CGGAAGA446450.013.9322834
TATGCCG121000.012.23550545-49
CGTATGC137150.010.64670545-49
CTCGTAT142800.010.215425540-44
TCTCGTA143250.09.96063840-44
GCCGTCT160400.09.14853350-54
ATGCCGT161450.09.1161145-49
TGCCGTC154650.08.31666250-54
TCGTATG159050.08.11405345-49
ATCTCGT185100.07.896611740-44
AGAGCAC826900.07.83775578
GAGCACA881550.07.38476379
CGTCTTC208350.07.237942750-54
GAAGAGC934500.07.12924246
CCGTCTT217150.06.978012650-54
CATCTCG215800.06.874020640-44
AGATCGG398550.05.8766947145
Produced by FastQC (version 0.11.5)