FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005014968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68486029
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC338050.017.508229
CGCCGTA89800.015.58478855-59
AGAGCGT394900.015.0610628
GATCGGA415750.014.6869351
ATCGGAA437350.014.1658322
TCGCCGT102400.013.76606355-59
TCGGAAG454400.013.5507233
GTCGCCG107100.013.28356855-59
AAGAGCG465800.012.9705347
CGGAAGA473650.012.9236154
GCCGTAT131550.010.93569255-59
TGGTCGC146350.09.94842250-54
GTGGTCG160250.09.18484650-54
CGTATCA160050.09.16058360-64
GGTCGCC137900.08.67944555-59
CCGTATC150350.08.095839560-64
TCTCGGT213500.07.300904345-49
GAAGAGC967250.06.7324796
CTCGGTG233400.06.54819945-49
CGGTGGT232650.06.51939450-54
Produced by FastQC (version 0.11.5)