FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32485123
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC163650.010.8981669
AGAGCGT185350.09.7396298
CGCCGTA44400.09.73251155-59
GATCGGA198450.09.4148771
ATCGGAA205450.08.9989622
TCGCCGT51650.08.7032855-59
TCGGAAG212700.08.6575273
CGGAAGA225200.08.4024214
AAGAGCG221400.08.382927
GTCGCCG59300.07.507153555-59
GCCGTAT61600.07.39163555-59
TGGTCGC68850.06.92896650-54
CGTATCA77450.06.08453860-64
GTGGTCG78600.06.014112550-54
CGTCGTG120000.05.8602314145
GCGTCGT118550.05.6872935145
GACGGTT83350.05.532278530-34
CCGTATC70850.05.362003360-64
AGATCGG202850.05.3609347145
TAGATCG75050.05.1197586145
Produced by FastQC (version 0.11.5)