FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48047683
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA79750.013.43996255-59
GAGCGTC265050.013.0623389
TCGCCGT90650.011.919003555-59
AGAGCGT302600.011.51262958
GATCGGA314850.011.3131081
GCCGTAT100750.010.66710255-59
ATCGGAA338100.010.5449672
TCGGAAG347850.010.26365953
GTCGCCG104000.09.97456155-59
CGGAAGA369400.09.7039754
AAGAGCG372550.09.5247897
TGGTCGC118600.09.35178350-54
GTGGTCG125950.08.82885850-54
CGTATCA122000.08.69129560-64
CGGTGTA125450.08.62275635-39
ACCGGTG141950.07.731784335-39
AGCGTCG217300.07.70190059
CACCGGT141650.07.48447835-39
TCTCGGT168300.06.93097145-49
CCGGTGT159050.06.90954635-39
Produced by FastQC (version 0.11.5)