FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79525904
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA530250.025.0263631
ATCGGAA556500.024.0269322
TCGGAAG569000.023.4024243
CGGAAGA598850.022.2359184
TATGCCG186650.015.78546345-49
CTCGTAT212500.013.55734240-44
AGAGCAC1013700.013.42913158
TCTCGTA211500.013.35408840-44
CGTATGC211600.013.25949345-49
GAGCACA1081950.012.7361079
ATGCCGT233700.012.45852445-49
GAAGAGC1129700.012.1849466
GCCGTCT239550.011.70653250-54
TCGTATG233000.011.56249145-49
TGCCGTC237900.011.28183950-54
ATCTCGT253600.010.794147540-44
CGTCTTC291850.010.24961350-54
CCGTCTT291200.09.819365550-54
AAGAGCA1554350.09.1171647
GGAAGAG1783000.08.0659275
Produced by FastQC (version 0.11.5)