FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005015107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50313969
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA208200.016.5733011
ATCGGAA224050.015.4672452
TCGGAAG236550.014.5575913
CGGAAGA260050.013.4093284
TATGCCG75850.010.28412245-49
CTCGTAT90150.08.65288140-44
TCTCGTA90400.08.24401740-44
CGTATGC92650.08.07504345-49
ATGCCGT111300.07.347244345-49
TATCTCG99100.07.06671640-44
AGAGCAC543950.06.7569218
TCGTATG104950.06.74183245-49
GCCGTCT123850.06.41525650-54
GAGCACA596450.06.33232939
GAAGAGC609300.06.2344766
TGCCGTC121500.06.10974550-54
AGATCGG207500.06.0441194145
ATCTCGT123000.06.00007740-44
GTCACGT143150.05.763272325-29
CGTGCTT149400.05.580781530-34
Produced by FastQC (version 0.11.5)