FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005015144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34542482
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA46450.010.23392355-59
TCGCCGT58550.08.41600655-59
GAGCGTC209750.08.1188649
GCCGTAT62950.07.850777655-59
AGAGCGT237850.07.22062168
GTCGCCG67950.07.08113155-59
TGGTCGC76600.06.565307650-54
CGTATCA78850.06.54342660-64
GATCGGA261950.06.4424651
ATCGGAA272750.06.29707342
TCGGAAG277650.06.2378643
CGGAAGA289400.06.0346014
AAGAGCG289700.05.9783077
GCGTCGT160600.05.9560432145
GTGGTCG87750.05.863211650-54
CGTCGTG159800.05.6684284145
AGATCGG275200.05.6350102145
AGCGTCG173450.05.598348145
TCGTGTA163050.05.5109987145
GCGTCGG31454.624737E-65.0691066145
Produced by FastQC (version 0.11.5)