FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005015212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32677653
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC154300.024.5230949
GATCGGA177750.022.395851
AGAGCGT177800.021.6487838
ATCGGAA189100.020.783882
TCGGAAG191550.020.4011383
CGGAAGA212850.018.4942234
AAGAGCG211150.018.4013797
CGCCGTA57550.014.86412755-59
TCGCCGT65350.013.28966255-59
GTCGCCG74250.011.54047655-59
GCCGTAT77400.011.29559455-59
TGGTCGC86100.010.23872950-54
GTGGTCG92150.09.72386150-54
CGTATCA91850.09.58170160-64
GACGGTT90050.09.57942430-34
GAAGAGC443900.09.2918846
CCGTATC83200.09.23603760-64
TGACGGT104950.08.44043630-34
GGTCGCC89300.08.31287455-59
TCTCGGT120800.07.765516345-49
Produced by FastQC (version 0.11.5)