FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409281950
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG25322980.6187172436996061TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4315300.0107.27951
TCGGAAG4466350.0103.4890062
CGGAAGA4710600.097.936323
AGAGCAC7083400.065.2175757
AGCACAC7381950.062.5432059
GAAGAGC7503700.061.698325
GAGCACA7489350.061.6814358
AAGAGCA9755100.047.534586
GGAAGAG10672050.043.683114
TATGCCG3856850.024.19641545-49
CTCGTAT3448650.023.55923840-44
TCTCGTA3463700.023.3710640-44
CGTATGC3979000.023.22550845-49
ATGCCGT4033750.022.88618345-49
TTACTCG4103100.022.42528730-34
TACTCGA4143450.022.35389135-39
GCCGTCT4074200.022.3034750-54
TGCCGTC4069100.022.28649345-49
TCGTATG3609700.022.05423440-44
ATCTCGT3666100.022.04162240-44
Produced by FastQC (version 0.11.5)