FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409281950
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC28418780.6943570318700837Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5254150.087.078229
GAGCGTC5545400.082.5142448
ATCGGAA5816700.080.130661
TCGGAAG5995300.077.639242
AGAGCGT5930350.077.280057
CGGAAGA6259250.074.2470863
AAGAGCG6520550.070.9548656
GAAGAGC9037500.051.482785
GGAAGAG12666800.037.0957344
CGCCGTA4205600.021.0498755-59
TCGCCGT4253450.020.67809155-59
GCCGTAT4356550.020.33681955-59
GTCGCCG4709350.019.5801355-59
CCGTATC4453600.019.56975755-59
TGGTCGC4754150.019.55556150-54
CGTATCA4529600.019.34726760-64
GGTCGCC4894450.018.81638750-54
GTGGTCG4993550.018.6967150-54
GCGTCGT5045650.018.0941310-14
CGTCGTG5076200.018.02835810-14
Produced by FastQC (version 0.11.5)