FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495984647
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC37373750.753526348568608Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6452500.090.157169
GAGCGTC6806300.085.445068
ATCGGAA7067250.083.794431
TCGGAAG7306800.080.768932
AGAGCGT7258350.080.190927
CGGAAGA7601300.077.590463
AAGAGCG7992450.073.584326
GAAGAGC11053000.053.4959075
GGAAGAG15231850.039.1808474
CGCCGTA5267500.021.77316755-59
TCGCCGT5333900.021.4154155-59
GCCGTAT5447750.021.07298355-59
CCGTATC5546300.020.4047255-59
GTCGCCG5783200.020.35733255-59
TGGTCGC5850350.020.29092450-54
CGTATCA5650750.020.1420560-64
GGTCGCC6003350.019.57696350-54
GTGGTCG6099550.019.54251150-54
GCGTCGT6285850.018.66604810-14
CGTCGTG6324550.018.61143510-14
Produced by FastQC (version 0.11.5)