FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392559327
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC15114200.3850169633085804Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3700050.064.681599
GAGCGTC3961450.060.4655238
ATCGGAA4203700.058.30341
TCGGAAG4364100.055.969772
AGAGCGT4317700.055.6170777
CGGAAGA4591850.053.119133
AAGAGCG4885500.049.6308176
GAAGAGC7301950.033.591595
GGAAGAG10625950.023.453174
CGCCGTA2885550.018.45235455-59
TCGCCGT2935700.018.05615655-59
GCCGTAT3022850.017.62190855-59
GTCGCCG3251700.016.71114755-59
CGTATCA3183350.016.63234960-64
TGGTCGC3287450.016.59752550-54
GTGGTCG3470300.015.78273450-54
CCGTATC3108450.015.35801455-59
GCGTCGT3547950.015.17932410-14
CGTCGTG3580350.015.09510-14
TCGTGTA3664950.014.72559110-14
Produced by FastQC (version 0.11.5)