FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437111363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG11404060.2608959859046263TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2603550.083.695061
TCGGAAG2738250.079.4137042
CGGAAGA2996300.072.541583
AGAGCAC5511900.039.7330637
AGCACAC5870600.037.268299
GAAGAGC5962250.036.815775
GAGCACA5999450.036.5029148
AAGAGCA8421300.026.2857536
GGAAGAG9314550.023.9054134
TATGCCG2155550.020.55693245-49
CTCGTAT1934700.019.3840340-44
CGTATGC2274000.019.30423545-49
TCTCGTA1977750.018.88506140-44
ATGCCGT2383850.018.48287245-49
TACTCGA2411850.018.33479935-39
TTACTCG2394050.018.16401330-34
TGCCGTC2381800.018.12034645-49
GCCGTCT2411750.018.04551550-54
TCGTATG2102800.017.3300440-44
ATCTCGT2182100.017.24417940-44
Produced by FastQC (version 0.11.5)