FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437111363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC13720750.31389598078236186Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3505100.061.7203839
GAGCGTC3886600.055.6627358
ATCGGAA4056450.054.23571
AGAGCGT4144800.052.269097
TCGGAAG4216150.052.0730552
CGGAAGA4500900.048.7009163
AAGAGCG4736500.046.090826
GAAGAGC7457750.029.6394625
GGAAGAG11068850.020.3896474
CGCCGTA2508250.017.29213755-59
TCGCCGT2606250.016.62134655-59
GCCGTAT2685100.016.17858355-59
GTCGCCG2904400.015.3197555-59
CCGTATC2787850.015.23245755-59
CGTATCA2862800.015.0889360-64
TGGTCGC2974950.015.07051750-54
GTGGTCG3155650.014.27092150-54
GGTCGCC3094250.014.21359850-54
TCTTCGC3451200.013.00986930-34
GTCTTCG3464350.012.8872830-34
Produced by FastQC (version 0.11.5)