FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412755268
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC14764720.3577112430700703Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3830600.059.6168639
GAGCGTC4112850.055.6136938
ATCGGAA4503450.051.553361
AGAGCGT4552600.050.36127
TCGGAAG4613600.050.2038352
CGGAAGA4847900.047.7764663
AAGAGCG5127450.044.9950456
GAAGAGC7739450.030.1549875
GGAAGAG11368950.020.8526124
CGCCGTA2833300.016.42108255-59
TCGCCGT2890000.016.06229455-59
GCCGTAT3049950.015.33923455-59
GTCGCCG3060300.015.32714755-59
TCCGATC315150.014.8441983
CCGTATC3123850.014.70580655-59
TGGTCGC3228250.014.66752250-54
GGTCGCC3232400.014.43162650-54
CGTATCA3230750.014.40985560-64
GTGGTCG3429850.013.85146350-54
GCGTCGT3670900.012.70317210-14
Produced by FastQC (version 0.11.5)