FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455763166
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG18334560.40228261886349986TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3406550.096.113171
TCGGAAG3550400.091.951862
CGGAAGA3820000.085.383883
AGAGCAC6498900.050.4453627
AGCACAC6855800.047.794319
GAAGAGC6955300.047.2134135
GAGCACA6946150.047.180598
AAGAGCA9492300.034.8336756
GGAAGAG10399950.031.885624
TATGCCG2963100.022.44485545-49
CTCGTAT2752000.021.74566840-44
TCTCGTA2784300.021.40228740-44
CGTATGC3100200.021.27739145-49
ATGCCGT3212550.020.63841645-49
TTACTCG3209150.020.5248330-34
TGCCGTC3215850.020.28545845-49
GCCGTCT3236950.020.14561850-54
TACTCGA3293000.020.12029535-39
ATCTCGT2990550.019.94222640-44
TCGTATG2976650.019.77869240-44
Produced by FastQC (version 0.11.5)