FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417388700
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG6014810.14410572207632838TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1706550.064.212231
TCGGAAG1840000.059.5417862
CGGAAGA2053150.053.2786033
AGAGCAC4499700.024.8241637
AGCACAC4860850.023.006679
GAAGAGC4933250.022.6572195
GAGCACA4956100.022.516198
CGCGCAT1187600.018.95003535-39
TCGCGCA1269400.017.98812135-39
TCTCGCG1314700.017.62347230-34
TATGCCG1332650.017.52845-49
GCGCATC1335150.016.99914435-39
CTCGTAT1269450.016.2117940-44
CGTATGC1459800.015.92780745-49
TCTCGTA1289850.015.664309540-44
AAGAGCA7299250.015.5992356
CTCGCGC1458700.014.992290530-34
ATGCCGT1568550.014.94458145-49
CGCATCT1378850.014.64792735-39
GCCGTCT1598300.014.42192250-54
Produced by FastQC (version 0.11.5)