FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459314713
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1633100.047.877661
TCGGAAG1754550.044.5067562
CGGAAGA1968150.039.595473
AGAGCAC4637350.017.2726237
AGCACAC5032000.016.0372359
GAAGAGC5139400.015.7236645
GAGCACA5131350.015.70138
CGCGAAA1075350.014.85779935-39
TCCGCGA1117300.014.41674730-34
TATGCCG1198550.013.70679445-49
CCGCGAA1146400.013.49933730-34
CGTATGC1328100.012.3151745-49
CTCGTAT1128700.012.14376340-44
CTCCGCG1353400.012.12245230-34
GCGAAAT1377750.011.84400635-39
TCTCGTA1167000.011.75763740-44
ATGCCGT1480600.011.15542745-49
TGCCGTC1423550.011.05959445-49
GCCGTCT1469750.010.98719650-54
ACTCCGC1549950.010.69371630-34
Produced by FastQC (version 0.11.5)