FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005015352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459314713
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC4660580.10146811909332414Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2362400.032.1147279
GAGCGTC2676150.028.4363578
ATCGGAA2990500.025.8339081
TCGGAAG3137500.024.5680482
AGAGCGT3120900.024.4886387
CGGAAGA3384100.022.7164883
AAGAGCG3644600.021.0255556
GAAGAGC6520600.012.2302875
CGCCGTA1500400.010.38430855-59
TCGCCGT1577050.09.8722455-59
GTCGCCG1729650.09.27368755-59
GCCGTAT1749600.09.06352655-59
TCCGATC361300.09.0520323
TGGTCGC1929800.08.46886750-54
CCGTATC1832800.08.30954855-59
GGTCGCC1916150.08.19853150-54
CGTATCA1963600.08.07201560-64
GGAAGAG10415550.07.9992974
GTGGTCG2135650.07.774097450-54
TTCGCGG194950.07.14233061
Produced by FastQC (version 0.11.5)