FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005015391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316522
Sequences flagged as poor quality0
Sequence length20-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA22950.026.187111
TCGGAAG26950.022.5537552
ACGTGCG6050.019.1744215
CGGAAGA33850.018.1431433
CGTGCGC11354.3837645E-1010.8219456
TATGCCG17000.010.36301645-49
ACATCGG11550.002784466810.072796145
GTGCGCA17701.8189894E-118.8670687
TGCGCAC17007.1122486E-108.4292268
TATCGGA10101.08393E-48.1142331
CTCGTAT22100.07.942116740-44
TCTCGTA23300.07.41350840-44
CGACGCT4102.8885552E-97.39198825-29
ATGCCGT24900.07.29975945-49
CGTATGC22250.07.28941245-49
TGCCGTC23500.06.961174545-49
GCGCACA22752.7946953E-86.59872779
GCCGTCT25350.06.530794650-54
TCGTATG26350.06.50253240-44
AGAGCAC108800.06.2718637
Produced by FastQC (version 0.11.5)