FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005015392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005015392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316522
Sequences flagged as poor quality0
Sequence length20-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG13150.041.461259
GAGCGTC21400.026.4456468
ATCGGAA23250.024.95081
TCGGAAG26200.021.6142772
AGAGCGT28900.020.060297
CGGAAGA31300.018.301413
AAGAGCG36500.016.4506136
CGCCGTA11600.011.48899855-59
TCGCCGT14300.08.731132555-59
GTCGCCG14800.08.34137355-59
CGCACGT15500.08.238873530-34
GCGTCGT14150.07.99917310-14
CGTCGTG14800.07.927657610-14
GGTCGCC18150.07.78574250-54
TGGTCGC19300.07.68429150-54
GCCGTAT19000.07.67885255-59
GCGCACG19450.06.779781330-34
GTGGTCG22450.06.543772750-54
CCGTATC23800.06.248063655-59
GAAGAGC113450.05.77929455
Produced by FastQC (version 0.11.5)