FastQCFastQC Report
Wed 8 May 2024
EGAF00005026136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005026136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144245
Total Bases38.9 Mbp
Sequences flagged as poor quality0
Sequence length34-301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGAGCTGAAGAGGTTTCACATCTTACTCCGCCCCACACCCTGGGCGT1584410.98408956982911No Hit
AGAAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTG139999.705015771777184No Hit
GGTGGACTTATTTTGCCTCAGAGACCAAATGTGCTGAATTATGGATGGGA120958.385039342784845No Hit
CACACATTTTGGGAGGGTGTCTTTAGAAGCTGACCTGATAATGTGGGATG116008.041873201844085No Hit
CTCTATGGCTGGAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAG108697.535096537141668No Hit
TGGTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTA107827.474782488127837No Hit
GGTTCCTGCATTGTCATGGATTAAAACTGAGTAGGCAGTGGAAGATAAAT63124.375888245693091No Hit
GCTATGCTCCCACTACCTGCCCAGACAAAATCATTTGGCTGTACTATCTT44583.0905750632604247No Hit
GGCACGAATACAAGATGCAGCATTATAAATTTCTCATCACATAATGTTTA33762.340462407709106No Hit
GTTGCAGGAGAGCAGAGGACTCTCTAACTTTACTTCCACTCATTTGCTTT32982.2863877430760167No Hit
AGTGGGAGACTGTGGTTTGGGGAAAGTGGACTTTTTTAAATGCCTTGGGT31442.1796249436722244No Hit
TCTGAAAGTGGTTGCTGGTTCAGATAATGGCTTCTCACCAGTGTTTTTTT27461.903705501057229No Hit
GACCCTCAGTGTGACCAGTGAAGAAAACAGCCTTGTAAAAAAAAAAGAAA26521.8385385975250443No Hit
ATGGACTTTGAACCCAGGCCTCAATTCAGTGCTTTCTCCCTACCACACTG24961.730389268258865No Hit
ATAGTGCCTGGCATGCTTTGTAATAGTGTTGATATTATTATTTGCATCCC24331.6867135775936775No Hit
AAAATTTGATCCTGAACTCAATGATCTTTCTTTTTTTTTTACAAGGCTGT23741.6458109466532633No Hit
CAGATACCTATGGCAACATTTGGAAATTAATTAGAAATCTGAAATGTGGT19531.3539464106208188No Hit
CATCATCAACACCCTCATCTTCTTTCAAAAGCAAATGAGTGGAAGTAAAG19061.3213629588547264No Hit
GCTATGCTCCCACTACCTGCCCAGACAAAATCATTTGGCTGTGCTATCTT11010.7632846892439946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8810.6107664043814344No Hit
GTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTAAA8030.5566917397483448No Hit
GCTATGCTCCCACTACCTGCCTCCTCCCAAAACCACTTGATCCTTTGCCT6760.46864709348677597No Hit
CTCTATGGCTGGAAAGGTGGGAAGGGCCAGACCATGCAGTGAGGAATGGC5580.3868418316059482No Hit
TCTATGGCTGGAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAGT4190.29047800617005787No Hit
CTGAAAGTGGTTGCTGGTTCAGATAATGGCTTCTCACCAGTGTTTTTTTG3910.27106658809664114No Hit
AAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAAA3260.2260043675690665No Hit
CGTGAGCTGAAGAGGTTTCACATCTTACTCCGCCCCACACCCTGGGCGTT2950.20451315470206938No Hit
ACACATTTTGGGAGGGTGTCTTTAGAAGCTGACCTGATAATGTGGGATGT2850.19758050539013483No Hit
GGTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTAA2590.17955561717910498No Hit
AGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAAAC2570.17816908731671807No Hit
GAAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGA2100.14558563555062567No Hit
GTGGGAGACTGTGGTTTGGGGAAAGTGGACTTTTTTAAATGCCTTGGGTG1930.13380013172033692No Hit
GTTGCAGGAGAGCAGAGGACAAGAGAAAAACACCCCGGAAGCCAAGGGAA1600.1109223889909529No Hit
AAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAA1470.10190994488543798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)