FastQCFastQC Report
Wed 8 May 2024
EGAF00005026255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005026255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189962
Total Bases52.3 Mbp
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTG1980110.423663680104442No Hit
CCGTGAGCTGAAGAGGTTTCACATCTTACTCCGCCCCACACCCTGGGCGT1977710.41102957433592No Hit
GGTGGACTTATTTTGCCTCAGAGACCAAATGTGCTGAATTATGGATGGGA167158.799128246701972No Hit
CACACATTTTGGGAGGGTGTCTTTAGAAGCTGACCTGATAATGTGGGATG163188.590139080447669No Hit
CTCTATGGCTGGAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAG144607.61204872553458No Hit
TGGTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTA123276.489192575357176No Hit
GCTATGCTCCCACTACCTGCCCAGACAAAATCATTTGGCTGTACTATCTT86274.541434602710016No Hit
GGTTCCTGCATTGTCATGGATTAAAACTGAGTAGGCAGTGGAAGATAAAT85364.493530285004369No Hit
AAAATTTGATCCTGAACTCAATGATCTTTCTTTTTTTTTTACAAGGCTGT75733.9865867910424186No Hit
GTTGCAGGAGAGCAGAGGACTCTCTAACTTTACTTCCACTCATTTGCTTT70353.703372253398048No Hit
GGCACGAATACAAGATGCAGCATTATAAATTTCTCATCACATAATGTTTA57613.0327118055189985No Hit
GACCCTCAGTGTGACCAGTGAAGAAAACAGCCTTGTAAAAAAAAAAGAAA48822.5699876817468756No Hit
CAGATACCTATGGCAACATTTGGAAATTAATTAGAAATCTGAAATGTGGT44702.353102199387246No Hit
CATCATCAACACCCTCATCTTCTTTCAAAAGCAAATGAGTGGAAGTAAAG44072.319937671744875No Hit
ATGGACTTTGAACCCAGGCCTCAATTCAGTGCTTTCTCCCTACCACACTG34841.834051020730462No Hit
ATAGTGCCTGGCATGCTTTGTAATAGTGTTGATATTATTATTTGCATCCC34191.7998336509407145No Hit
AGTGGGAGACTGTGGTTTGGGGAAAGTGGACTTTTTTAAATGCCTTGGGT32791.7261347006243353No Hit
TCTGAAAGTGGTTGCTGGTTCAGATAATGGCTTCTCACCAGTGTTTTTTT20871.0986407807877363No Hit
CTGAAAGTGGTTGCTGGTTCAGATAATGGCTTCTCACCAGTGTTTTTTTG13100.6896116065318327No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12020.6327581305734831No Hit
TGGTATCGTAATGTACTGTCCCCAAGCAAACATTTAAAAAGCCAATTTTA10380.5464250744885819No Hit
GTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTAAA8730.4595655973299923No Hit
ATCATCAACACCCTCATCTTCTTTCAAAAGCAAATGAGTGGAAGTAAAGT5870.30900917025510366No Hit
TCTATGGCTGGAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAGT5770.3037449595182194No Hit
CGTGAGCTGAAGAGGTTTCACATCTTACTCCGCCCCACACCCTGGGCGTT5010.26373695791789936No Hit
AAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAAA4520.2379423253071667No Hit
AGATACCTATGGCAACATTTGGAAATTAATTAGAAATCTGAAATGTGGTC4520.2379423253071667No Hit
CTATGCTCCCACTACCTGCCCAGACAAAATCATTTGGCTGTACTATCTTT3570.1879323233067666No Hit
AAAATTTGATCCTGAACTCAATGATCTTTCTTTTTTTTTACAAGGCTGTT3470.18266811256988238No Hit
AGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAAAC3140.16529621713816448No Hit
GCACGAATACAAGATGCAGCATTATAAATTTCTCATCACATAATGTTTAA2930.1542413745907076No Hit
GTGGGAGACTGTGGTTTGGGGAAAGTGGACTTTTTTAAATGCCTTGGGTG2870.1510828481485771No Hit
ACACATTTTGGGAGGGTGTCTTTAGAAGCTGACCTGATAATGTGGGATGT2650.1395015845274318No Hit
GGTATCGTAATGTACTGTCCCCCAGCAAACATTTAAAAAGCCAATTTTAA2600.1368694791589897No Hit
CATCATCAACACCCTCATCTTCTTTCAAAAGCAACTGAGTGGAAGTAAAG2560.134763794864236No Hit
CAGATACCTATGGCAACATTTGGAAATTAATTAGCAATCTGAAATGTGGT2520.13265811056948232No Hit
GTGGACTTATTTTGCCTCAGAGACCAAATGTGCTGAATTATGGATGGGAG2400.12634105768522125No Hit
GAAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGA2160.11370695191669913No Hit
AAAGGCCCACACACTGTCCACTCTCCCGATACCATTTTACAGGTGGTGAA2100.1105484254745686No Hit
GACCCTCAGTGTGACCAGTGAAGAAAACAGCCTTGTAAAAAAAAAGAAAG1930.10159926722186544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)