FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005028390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124193271
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA159450.08.61350655-59
GAGCGTC777100.07.62965259
TCGCCGT190300.07.41529855-59
AGAGCGT882250.06.8024768
GCCGTAT221000.06.46395755-59
GTCGCCG235200.06.086033355-59
ATCGGAA1021950.05.90199952
GATCGGA992250.05.9008711
TCGGAAG1042450.05.82728773
AAGAGCG1055300.05.7763177
AGATCGG1034000.05.7636046145
AGCGTCG652850.05.6636925145
CGTCGTG592200.05.6560926145
CGGAAGA1097350.05.502594
GCGTCGT599100.05.4457297145
TGGTCGC267350.05.398387450-54
GTCGTGT641250.05.3252063145
CGTATCA280600.05.281313460-64
GTGGTCG294750.05.0983250-54
TCGTGTA621700.05.0145326145
Produced by FastQC (version 0.11.5)