FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21660225
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC7253.0559022E-1014.9989976
CACGACG8852.910383E-1113.9256225
CGACGCT7558.69295E-913.442817
TATGCCG43800.010.59294845-49
GATCGGA151350.09.2537941
ATCGGAA159450.08.9590712
CTCGTAT49200.08.95878440-44
TCGGAAG160650.08.8010413
CGGAAGA168000.08.6312714
CGTATGC51800.08.59309545-49
TCTCGTA50400.08.34272740-44
ATGCCGT54650.08.3306845-49
GCCGTCT60050.07.509537750-54
CCGTGTA68600.06.911390330-34
CGTCTTC71850.06.82112150-54
CGTGTAG68000.06.80179530-34
ATCTCGT63350.06.751744340-44
CCGTCTT74250.06.463940650-54
GTCACCG77550.06.169871325-29
ACACGAC21304.2540254E-55.7865994
Produced by FastQC (version 0.11.5)