FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005028462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115675564
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC486850.012.9992429
GATCGGA532450.012.1251621
CGCCGTA146850.011.14763355-59
ATCGGAA583850.011.1383762
AGAGCGT571550.011.1362448
TCGGAAG620850.010.5204313
CGGAAGA673050.09.6614114
AAGAGCG694850.09.3270437
TCGCCGT176150.09.3180855-59
GCCGTAT205400.08.30880655-59
GTCGCCG209500.08.07006755-59
TGGTCGC244700.07.116180450-54
GTGGTCG271400.06.54965750-54
CGTATCA262950.06.545222360-64
CCGTATC244300.05.501778660-64
AGCGTCG377900.05.33294259
TCTCGGT354750.05.247989745-49
GGTCGCC256750.05.20129955-59
Produced by FastQC (version 0.11.5)