FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25255056
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA159950.025.1069031
ATCGGAA170200.023.7302282
TCGGAAG174950.023.0809573
CGGAAGA185350.021.74664
TATGCCG66050.015.78330445-49
CTCGTAT73550.014.351240-44
TCTCGTA73750.013.4865740-44
ATGCCGT82450.013.013164545-49
AGAGCAC317350.012.9068158
CGTATGC73200.012.41933545-49
GAGCACA338450.012.3163629
GAAGAGC359450.011.5161356
ATCTCGT88000.010.84133340-44
GCCGTCT83200.010.83963450-54
CGTCTTC97950.010.820827550-54
TGCCGTC80450.010.14681950-54
CCGTCTT100050.010.04300550-54
TCGTATG85400.09.60949245-49
AAGAGCA488250.08.6118187
GTATGCC135550.07.78706445-49
Produced by FastQC (version 0.11.5)