FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13117223
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA42000.014.77452555-59
TCGCCGT45550.013.43207555-59
GCCGTAT46850.013.12125355-59
GTCGCCG50400.012.34087155-59
CGTATCA52650.011.84112860-64
TGGTCGC56600.011.09152250-54
GTGGTCG58050.010.91437550-54
GAGCGTC197700.010.708619
AGAGCGT214000.09.9613978
AAGAGCG242200.08.9577747
TCTCGGT72100.08.92835345-49
GATCGGA250650.08.5843391
ATCGGAA256200.08.5274622
TCGGAAG253600.08.4999823
CTCGGTG77500.08.47461845-49
CGGAAGA257600.08.4238954
CGGTGGT77700.08.32211150-54
CCGTATC51900.08.04540960-64
GGTCGCC56450.07.550964455-59
GCGTCGG11702.9788006E-47.4357104145
Produced by FastQC (version 0.11.5)