FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58610156
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA421350.016.676211
ATCGGAA443200.015.9490432
TCGGAAG450400.015.6133633
CGGAAGA462100.015.2180454
TATGCCG134400.013.680016545-49
ACGACGC22100.013.12037856
CTCGTAT154250.011.6174940-44
CGACGCT24350.011.61033257
CGTATGC153250.011.58104145-49
TCTCGTA151700.011.53561440-44
ATGCCGT168350.010.6887145-49
GCCGTCT175000.010.06760550-54
AGAGCAC762150.09.6537688
CCGTGTA191200.09.629909530-34
CACGACG28450.09.4275185
CGTGTAG190200.09.35277530-34
GAGCACA810050.09.0650619
ATCTCGT185700.08.97852440-44
CGTCTTC212200.08.92453850-54
GAAGAGC860850.08.6144346
Produced by FastQC (version 0.11.5)