FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005028527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115693036
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA758000.020.6165391
TCCGATC90900.018.8231093
ATCGGAA838600.018.6223342
TCGGAAG855150.018.2364943
CGGAAGA888500.017.6661014
TATGCCG319400.015.14324445-49
CCGATCT127600.013.5796164
CTCGTAT354250.013.35878240-44
CGTATGC355150.013.34537845-49
TCTCGTA343250.012.9970140-44
ATGCCGT387500.012.25744645-49
GCCGTCT394800.011.82849350-54
TTCCGAT153500.011.52456952
ATCTCGT405950.010.88958740-44
AGAGCAC1510050.010.7052348
CGTCTTC460850.010.50128850-54
CCGTCTT467050.010.17563350-54
GAGCACA1611400.010.1263929
GAAGAGC1686550.09.7452496
CTTCCGA198800.09.3214361
Produced by FastQC (version 0.11.5)