FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20495128
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCA21250.011.2604973
CGGCGAC20150.011.1908951
GATCGGA183900.09.7699741
ATCGGAA188050.09.6402262
TCGGAAG195300.09.3933563
TATGCCG51850.08.94823545-49
CGGAAGA203550.08.9057694
GGCGACC28100.08.5158542
CGACCAC28650.08.0989354
CTCGTAT58400.07.99435440-44
TCTCGTA59850.07.58264140-44
ATGCCGT63400.07.478157545-49
CGTATGC60850.07.29116545-49
AGAGCAC291500.06.46771868
GCCGTCT72600.06.151045350-54
GAGCACA314850.06.1492759
ATCTCGT73700.06.07898940-44
GAAGAGC326300.05.97639376
CCACCGA43750.05.96524767
ACCGAGA45100.05.78818379
Produced by FastQC (version 0.11.5)