FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20495128
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC162100.010.1483269
CGCCGTA40350.09.51889255-59
AGAGCGT178400.09.465158
GATCGGA191700.09.0937751
ATCGGAA196000.08.8369392
CGGAAGA211250.08.5763244
TCGGAAG203900.08.5656833
AAGAGCG210450.08.3335237
TCGCCGT49750.07.98254955-59
GCCGTAT50200.07.853248655-59
CGTATCA57950.06.903481560-64
GTCGCCG59700.06.82206955-59
TGGTCGC64050.06.24581550-54
GTGGTCG68300.06.02693950-54
CCGTATC58100.05.887736360-64
GAAGAGC342950.05.8745716
GCGTCGG23352.880551E-55.586889145
GACGGTT76300.05.16716630-34
TCTCGGT88300.05.15422345-49
GGTCGCC69650.05.139962755-59
Produced by FastQC (version 0.11.5)