FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21446175
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC18500.017.248163
CCGATCT23800.013.4038084
CGGCGAC11901.8189894E-1212.2366361
TATGCCG40100.012.04064945-49
TTCCGAT29800.010.9512092
CTCGTAT47950.010.52294840-44
CGTATGC51200.09.11876345-49
TCTCGTA48650.08.97078640-44
ATGCCGT56000.08.82909945-49
GATCGGA183550.08.72664451
ATCGGAA188850.08.601952
CTTCCGA38950.08.5986321
TCGGAAG190400.08.4937553
CGGAAGA202100.08.14351754
GCCGTCT56900.07.975940750-54
GCGACCA17302.0992411E-77.54550363
CGTCTTC69750.07.234106550-54
ATCTCGT61550.07.067080540-44
GGCGACC19552.139277E-77.0481112
CCGTCTT70500.06.807516650-54
Produced by FastQC (version 0.11.5)