FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21446175
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA30800.014.59230655-59
TCGCCGT36650.012.26311255-59
GTCGCCG40650.011.0564155-59
GCCGTAT42900.010.91586655-59
GAGCGTC143800.010.4386749
TGGTCGC47200.09.95196650-54
AGAGCGT158850.09.6325058
GTGGTCG52000.09.03332350-54
CGTATCA52000.08.81034460-64
GATCGGA188800.08.4035921
AAGAGCG184800.08.3200237
TCGGAAG197050.08.1026433
ATCGGAA195100.08.0706572
CGGAAGA207550.07.7589494
AGGTCGT61700.07.51908635-39
CGTCGTG107650.07.205378145
GGTCGTG69050.07.05465635-39
TCTCGGT68950.06.98072645-49
GCGTCGG16304.0612817E-56.670996145
GGTCGCC49050.06.443627455-59
Produced by FastQC (version 0.11.5)