FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56713646
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC39100.012.6085243
TATGCCG123450.012.26180245-49
GATCGGA406950.011.1747661
CTCGTAT139250.010.9330140-44
ATCGGAA434200.010.6702972
TCGGAAG438800.010.5576293
CGTATGC141050.010.30005545-49
CGGAAGA458400.010.074584
TCTCGTA137950.09.97447840-44
CCGATCT57600.09.8175754
ATGCCGT156900.09.8140145-49
GCCGTCT161750.08.86536950-54
ATCTCGT170500.08.09577140-44
CGTCTTC192200.07.91346950-54
TTCCGAT65650.07.73087642
CCGTCTT198150.07.514864450-54
AGAGCAC749100.06.49384938
GAGCACA800500.06.23086749
TCGTATG163750.06.13616245-49
TGCCGTC158550.06.12707750-54
Produced by FastQC (version 0.11.5)