FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17314458
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC95850.019.5132779
GATCGGA110350.018.2991051
AGAGCGT103600.018.1934998
ATCGGAA117500.017.1546172
TCGGAAG119750.016.8293323
CGGAAGA126900.015.7097284
AAGAGCG122750.015.6506377
CGCCGTA29800.015.61764855-59
TCGCCGT34300.013.61095555-59
GCCGTAT38250.012.31909455-59
GTCGCCG40050.011.69302555-59
TGGTCGC46300.010.61523250-54
GTGGTCG48950.010.27750350-54
CGTATCA46150.010.02240460-64
AGGTCGT50650.09.18903735-39
GGTCGTG55950.08.62955935-39
TCTCGGT64100.08.43667145-49
CCGTATC44450.08.28542760-64
GAAGAGC245050.08.0758696
GGTCGCC47600.07.73666255-59
Produced by FastQC (version 0.11.5)