FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30369300
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC173000.024.168279
AGAGCGT192600.021.9721438
GATCGGA193650.021.9422471
ATCGGAA207500.020.3942182
TCGGAAG217500.019.6560943
AAGAGCG223750.019.1075427
CGGAAGA232200.018.4741274
CGCCGTA54900.016.78994455-59
TCGCCGT64400.014.29066255-59
GCCGTAT71250.013.038855-59
GTCGCCG72750.012.96917855-59
TGGTCGC78650.012.05113350-54
CGTATCA83350.011.44167960-64
GTGGTCG86000.011.20655450-54
GAAGAGC430950.010.5091846
GGTCGCC80900.010.44442555-59
CCGTATC81950.010.25766560-64
TCTCGGT111950.09.02301645-49
CATTCGC112600.08.85514130-34
ATTCGCT111150.08.85331230-34
Produced by FastQC (version 0.11.5)