FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16236942
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT201470.12408124633320733TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA107400.033.0883641
ATCGGAA117550.030.2870162
TCGGAAG119750.029.9724313
CGGAAGA123750.028.943784
TCCGATC13650.024.435293
TATGCCG50050.017.75776345-49
TTCCGAT20550.017.6423242
AGAGCAC213400.016.7475978
CTCGTAT54500.016.0950640-44
GAGCACA232150.015.9258079
CGTATGC55050.015.90784945-49
TCTCGTA54200.015.64913340-44
CCGATCT20950.015.5740674
GAAGAGC236500.015.4489646
ATGCCGT59950.014.63180945-49
GCCGTCT61400.014.23940350-54
ATCTCGT65050.013.17267340-44
CCGTCTT72600.012.36223150-54
TCGTATG59000.012.311686545-49
CGTCTTC73400.012.26699950-54
Produced by FastQC (version 0.11.5)